With the continuous development of various technologies, optical microscopy becomes more and more important, and plays an important role in life science, material science and other fields.
There are many kinds of optical microscopes, such as ordinary optical microscope, fluorescence microscope, phase contrast microscope, inverted microscope, laser confocal scanning microscope, dark field microscope, polarizing microscope and so on. When using light microscopes, the accurate, rapid and stable movement of samples is very important for the study of samples.
In various optical microscopy systems, without the support of nano positioning system, the microscopic accuracy often cannot reach the requirements and the scanning speed is slow. Therefore, nano motion control systems are used in many microscope systems. Even if there is no microscope equipment pre-equipped with nano motion control system, it can be built by itself to add nano positioning system.
With mature technology and experience in piezo nano motion and control systems, CoreMorrow has equipped a variety of piezo nanosample stages, piezo objective scanners and piezo controllers for various optical microscope systems.
Application of CoreMorrow Piezo Objective Scanner in Optical Microscopy
Cold Atom Microscope
An atomic microscope uses a very sharp probe about the size of a few atoms at the top to scan the surface of the object under observation to create an image. Cold atom microscopy (cold atom microscopy) is a technique in which samples are imaged after being momentarily frozen in a vacuum.
CoreMorrow piezo objective scanner has a cryogenic vacuum version and can operate in a cryogenic vacuum environment.
Automatic Cell Imaging System
Automatic cell imaging system is a multi-dimensional and high-resolution digital microscopic imaging and analysis instrument, which can easily realize the imaging and biological analysis of low-density cells and tissues in porous plates, including cell counting, unlabeled cell counting, neurite tracking, etc. It has important applications in new drug development, cell biology research, histopathological analysis, environmental monitoring and other fields.
In this system, the adaptive continuous focus is achieved by the P72 series piezo objective scanner system. The piezo objective scanner can achieve a focusing range of 100μm, a resolution of 2.5nm, and a fast focusing animal lens, thus achieving automatic cell imaging.
Fully automatic microscopes are ideal for industrial scanning/inspection, metallography, laser cutting and other applications. They are also based on optical systems where light is struck onto the surface of the object to be observed or processed for imaging or laser processing (lithography).
The P76 piezo objective scanner has a large load capacity of 500g, which is suitable for fast scanning and focusing of heavy duty objective lenses.
Biological microscope is a precise optical instrument used to observe transparent or translucent objects such as biological cells, biological sections, bacteria and living tissue culture. The integrated of CoreMorrow piezo objective scanner in the biological microscope can greatly improve the focusing accuracy to nanometer.
Material Composition Analysis
Material composition analysis is the analysis of unknown substances or unknown components, through the analysis we can determine the composition of the sample and component content. The P76 series have integrated large-load piezo objectives scanner, which can bear the lens of heavier objects and provide nano-level focusing accuracy.
Application of Piezo Nanoscale Sample Table in Optical Microscopy
Structured Light Tomography 3D Imaging Technique
Structured light 3D imaging is to obtain the 3D shape of the surface of objects and scenes through non-contact, and reconstruct the surface of samples. In the reconstruction process, energy is radiated to the surface of the measured object, and the three-dimensional shape is reconstructed by collecting the reflected energy.
The imaging system is integrated with the CoreMorrow P11 piezo nanopositioning stage, providing rapid nanoscale scanning for structural tomography.
Pictured below is a bio-microscope with an integrated CoreMorrow P79.XYZ50S piezo sample stage, providing sample with an XYZ triaxial 50μm precision scanning range. And the sample can be rapidly focused and scanned in milliseconds.
Multi-head Objective Microscopy
In the observation process of some applications, a microscopic system with multiple object lenses will be used. The low-power lens will be used first to find the object to be observed, and then the high-power lens will be used for high-precision observation. In some applications, in order to observe cells and tissue structures of different sizes, different magnification lenses are also switched.
A microscope system with multiple object lenses and converters will increase the load weight of the piezo nanopositioning system. The CoreMorrow P12 Series one to three dimensional piezo scanner with load bearing capacity up to 800g or higher can be used for multi-lens fast nanoscale focusing. The switching of the object lens does not affect the Z-direction focusing of the piezo nano-positioning stage.
Biological Microscopic Imaging
P78.Z200 series piezo scanning table, whose core moves in the Z direction, is applied to the Z-direction focusing of biological microscopic system. To facilitate sample integration, a groove is integrated in the hollow part of the P78.Z200 so that the sample can be mounted and placed in the central through-hole position.
Fluorescence Microscopic Imaging
Fluorescence microscope is a kind of microscope equipment for product research by observing the fluorescence of objects. It is an important means and tool for carrying out microscopic life science research. This technology can be used to observe the fine structure of organisms and dynamically track the life activity processes of different scales such as tissues, cells, nuclei, proteins and small molecules in organisms, and has been applied in many different fields. Such as biological science, aerospace science and technology, educational institutions, etc.
Fluorescence microscope uses short wavelength light (excitation light) to irradiate the sample, so that the sample is excited by high energy, and produces long wavelength fluorescence (emission light), which is used to observe and distinguish the composition and position of fluorescent substances in the sample. Through the fluorescence microscope to see the fluorescence image morphological features and fluorescence color, brightness and so on to judge and record the image.
P12.XYZ piezo scanning table provides one to three dimensional nano motion, which can adjust the focus of the sample and also adjust the horizontal field of vision.
Several Z-axis and XYZ Piezo Sample Platforms Designed by CoreMorrow
In the optical microscope system, because of the different structure, the nano-motion positioning platform will have different structure and parameter requirements, CoreMorrow has a variety of piezo nano-positioning platform and controller standard products, and custom designed products according to user's specific application environment.
The following are some piezo sample platform for selection reference.
Closed/open loop resolution：5.5/2nm
You are welcomed to contact CoreMorrow for more detailed technical parameters and solutions!